Cloned (Comment) | Organism |
---|---|
gene GMK, expression in Escherichia coli strain BL21(DE3) | Arthrobacter sp. |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, hanging drop vapour diffusion method, cyrstallization drop on a silconized coverslip by mixing of 0.003 ml protein solution consisting of 10 mg/ml protein in 10 mM glucose, and 10 mM potassium phosphate, pH 7.0, with an equal volume of mother liquor composed of 2.0 M ammonium sulfate, 2% v/v PEG 400, and 0.1 M HEPES, pH 8.0, cyrstals are soaked in several heavy atom derivative solutions containing 1 mM of UO2Ac2, 10 mM AgNO3, 10 mM GdCl2, 1 mM HgCl2, 10 mM K2Pt(CN)4, 2 mM TmCl2, or 1 mM EuCl3 in 0.1 M Tris-HCl at different pH-values, dependent on the heavy atom salt, for 20-60 min at 20°C, X-ray diffraction structure determiination and analysis at 3.0 A resolution, modeling | Arthrobacter sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(phosphate)n + D-glucose | Arthrobacter sp. | - |
(phosphate)n-1 + D-glucose 6-phosphate | - |
? | |
(phosphate)n + D-glucose | Arthrobacter sp. KM | - |
(phosphate)n-1 + D-glucose 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter sp. | - |
gene GMK | - |
Arthrobacter sp. KM | - |
gene GMK | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli in several steps | Arthrobacter sp. |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(phosphate)n + D-glucose = (phosphate)n-1 + D-glucose 6-phosphate | glucose and phosphate substrate binding site structures | Arthrobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(phosphate)n + D-glucose | - |
Arthrobacter sp. | (phosphate)n-1 + D-glucose 6-phosphate | - |
? | |
(phosphate)n + D-glucose | - |
Arthrobacter sp. KM | (phosphate)n-1 + D-glucose 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | structure modeling of the C-terminal and N-terminal domains, and large and small domains, respectively | Arthrobacter sp. |
Synonyms | Comment | Organism |
---|---|---|
GMK | - |
Arthrobacter sp. |
inorganic polyphosphate/ATP-glucomannokinase | - |
Arthrobacter sp. |
More | the enzyme belongs to the poly(P)-dependent kinase family | Arthrobacter sp. |
poly(P)/ATP-glucomannokinase | - |
Arthrobacter sp. |